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Eukaryotic DNA Replication · Acedra
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Eukaryotic DNA Replication
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0:00
{\rtf1\ansi\ansicpg1252\deff0\nouicompat\deflang1033{\fonttbl{\f0\fnil\fcharset0 Arial;}} {\colortbl ;\red0\green0\blue0;} {\*\generator Riched20 10.0.18362}\viewkind4\uc1 \pard\cf1\fs24 Tutorial from Salisbury and talk about the replication is a completely caucus process of application compared to the so you can only thing the prokaryotic and eukaryotic system differs a lot while transcription or translation of
0:38
comparison, there are not that different button extorted replicas is more complex is the eukaryotic DNA is more complex is made with so much material so information right is composed of important genes and the things which are not putting for any put but it is all present in the DNA of the genome is huge, so application. This is the first lecture regarding the replication is part of the database is probably the most important part that you need to understand
1:14
very, very well. So for a basic idea about the more the mean simply making a copy of the replicas of the semi-Conservative application constant use as a template don't stamp template strand is produced utilising the constant and both the stands are utilised as a template to make to your daughter stands to gain articles can be found for one day is true for good reputation and there are a few important things in the proteins that the papacy eukaryotic
2:05
DNA replication memories which actually is involved in the job right and signs on for PETA, there epsilon and the different functions to talk about that the proteins which are also involved in the process of TA. An open top job is like a single standard binding protein to see which act as a camp application can include a protein that helps to load the PETA onto the template DNA so that the DNA synthesis and continuous. So in this case
2:33
of's RFC on e-zine recognition complex complex single structure single subunit proteins, most of them are my disabilities to submit complex is a protein, but it's an comprised of policy of the replication NCM's complex, so this is important very crucial in the process of also proteins ICT to also produce ICC proteins which we saw in case of cell cycle regulation and, the CTK or cycle -dependent kinase and TDK which is PDF for dependent district is
2:52
a very important part of the DNA replication to shift from the joint visit to the experts so that he can does the job they take the job of a is the time from this so's and complicated topics about the reputation the basic litigation remains the same properties five replicating DNA base pairs are that DNA is not free. I like the prokaryotic negative and not wrapped around the store is wrapped around. Access to the under this is important application
3:09
unwrap from the store completely and that making a new version. We going to require nucleosome histone proteins are also producing the and some escorting protein which is still pretty cytosol inside the nucleus complex and inside the nucleus size DNA onto the histone of the additional, which is not present, so she about the creation process at the start process of the tea replication in's starcrossed process will be using different proteins involved
3:34
in the process and what is that is making all the companies which are important for the initial purpose on the preparation, the. Actually, DNA replication in place inside the set in space is provided by the get-together because the presence of the and the city is high or actually dictates the process will continue to the next case or not, the producer CEO of CNC in like a split jealousy leads to a signal so that the application for different
4:14
stages so that the patient pre-initiation complex and second initiation steps of initiation process. If you're talking about seizure of eukaryotic replication and initiation here. Most of the protein synthesis and also included is the most proteins are utilised and the signal is received from CTK that provides the said. Now it's time to move from concentration to the CTK concentration is low, as this important job that no formation formation of pre-initiation
4:40
complex and is Activision activation of existing initiation complex pharmacy or the formation of pre-initiation complex C also activate the existing initiation of precision complex and converted into an from June to best logo that the formation of pre-initiation complex, but it prevents the activation of that copy simply activities the signals the conversion of the existing initiation complex complex formation of new releases and complex low
4:58
the formation of new process application does gets the signal activation CDs are prepared and considerations are from Japan to assess the consideration activate the existing initiation complex complex and. This process expressing a space in/complexes for complexes form application and properly talk about the steps of this initiation of this formation step of the process of application UK but before even before the application is submitted by CDA and TDK. Thus
5:24
think that not's different types of like. There are different types of solid as 123 architecture. Now the basic, but also because the research is going on so satisfactory that the job has two different kind of surveys jobs primer. It also adds us are further action is also done by the polymerase in the states legalised by the only in the process of repair. This is only for sums is this the application replication mitochondria might, and which
5:50
are involved in the process of actual polymerisation of the day by the lanky lagging strand synthesis in the Livingston Livingston synthesis. This can synthesise both released in situation when is okay for the and that it's has the capability of okay, you may find this is not all that is the strongest since it is not the litigation topics on the lines and synthesis of the leading strand synthesis and treatment of DNA synthesis and Livingston lagging strands
6:29
are different. Similarly, the Livingston synthesis is done continuously without any sort of gap or any fragments standardised and synthesis than small fragments are produced Okazaki facts and is another interesting idea for the much lead for the much smaller thus important difference between prokaryotic and application idea section of the the basics of the process of application and watching upon the name of this putting is going to come in the future,
7:04
so I to talk about the starting that means the formation of precision complex, and then the formation of initiation complex. Okay, so my mood is clear and also the name of some proteins is very difficult to put the name of the pudding is in the mind of an experience I very clearly mere individual. Each of is to understand the process of initiation of DNA replication. You can see what happens exactly replication initiation see the so
7:50
there is the origin of application is a proper application of soldier and his origin of replication site must be recognised by putting skills that is origin to commission contracts are complex, while the origin recognition region of the that the situation even start this process, you know that making the DNA really isn't this why the operatives are producing the already producing the sharpest and the prerecession complexes performs talking about
8:37
complex on this heavy caseload politics because normal in this case, we need to have that the hilly case since complex scripts that NASA is a similar job, they will only serve our between your two strands of the DNS through the separating distance from so includes six is here. CDC six ANSI C, the one NCM proteins and is now loaded start on why stands power metre. This structure a problem for me at this point this because replication origin
9:15
one is formed towards opposite direction. One crop top and in the initial site bubble visible in many different places of the complete length of the and now the template DNA strand the places are open and mining idea that the which the and versatile because it can act as the primary reasons I gets the primer sequences, so this is checks and concentration of the cell cycle sample at this moment the complex of proteins that are activated that this mission
10:24
in this pre-initiation complex complex, but what if I sense this context that the SS begins at this point. Once comment by and the jobs of this book is there an recruit and if, despite mother's friends so. The nucleotide sequences here processes the same and this I extension also done here as well, either in should all be loaded and exactly the same concept as I see that replicates are the strands to separate the enzymes. The two different standards confusion
12:12
stamp is involved in the synthesis of this is my standard lengths and sites by by the end, so this is how onto the destination stands there. See RFC. This is the replication factor in the job interaction and the region between the prime and the and this is important so is that this and see all factors junctions between sequences, which is extended by and this is a pretty easy application. We saw camp stands the template DNA and keep
13:22
on nucleotide sequences and increases the processability of the application capability of the polymers with this this answer or application factors see helps to in the stands this kind of like structures may not be able to stage of the recipients from the was's time for the movement and also stand stand. This is with, but is so different from places stands to find out that I'm not multiple primer contains multiple prime to cut primer, so I need to and
14:33
so sitting, but can be done by different methods which will talk in the process of termination letter, but, so excellent ties glossy nucleotides and their destination of the newly synthesise extracts. This is the initiation of discourses of SK and states this thing, stop, so that is put free to be utilised for dinner at that point the different proteins in plenty of proteins involved in the decision process of the replication beseeched the friends subscribe to the channel to get
19:04
the next part of this video that is the question of eukaryotic DNA replication as the termination of the details. What is the application series. Thank you receive in the next\par }
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